This book is an attempt to reveal the chemical concepts that rule the biological action of metalloproteins. The emphasis is on building up an understanding of basic ideas and familiarization with basic techniques. Enough background information is provided to introduce the field from both chemical and biological areas. It is hoped that the book may be of interest to workers in biological sciences, and so, primarily for this purpose, a brief survey of relevant properties of transition metals is presented.
The book is intended for undergraduates and postgraduates taking courses in coordination chemistry and students in biology and medicine. It should also be a value to research workers who would like an introduction to this area of inorganic chemistry. It is very suitable for self-study; the range covered is so extensive that the book can serve as a student's companion throughout his or her university career, At the same time, teachers can turn to it for ideas and inspirations.
The book is divided into seven chapters and covers a full range of topics in bioinorganic chemistry. It is well-illustrated and each chapter contains suggestions for further reading, providing access to important review articles and papers of relevance, A reference list is also included, so that the interested reader can readily consult the literature cited in the text.
It is hoped that the present book will provide the basis for a more advanced study in this field. Joseph J. Stephanos Anthony W, Addison

Preface ix

1 Introduction 1

Proteins: Formation, Structures, and Metalloproteins 4

References 28

2 Alkali and Alkaline Earth Cations 31

References 67

3 Nonredox Metalloenzymes 71

Carboxypeptidases 75

Carbonic Anhydrase 84

Alcohol Dehydrogenase 88

References 91

4 Copper Proteins 95

Introduction 95

Electronic Spectra of Copper Ions 96

ESR Spectra of Copper Ions 105

Copper Proteins 117

Plastocyanin 119

Azurin and.Stellacyanin 127

Superoxide Dismutase 131

Hemocyanin 135

Ascorbic Oxidase 139

References 142

5 Iron Proteins 147

Introduction 147

Electronic Spectra of Iron Ions 148

Mossbauer Spectroscopy of Iron Ions 155

ESR Spectra of Iron (III) 161

Iron Bioavailability 166

Siderophores 171

Iron Storage and Transfer Proteins 184

Ferritin 184

Transferrin 187

Dioxygenase Iron Proteins 195

Iron-Sulfur Proteins 207

Rubredoxin 207

Ferredoxins 212

2Fe-2S Ferredoxins 212

4Fe-4S Ferredoxins 221

Aconitase 226

Hydroxylases 229

Hydrogenases 232

Nitrogenases 240

Binuclear Iron Proteins 251

Hemerythrin 253

Ribotide Reductase, Purple Acid Phosphate, and Methane Monooxygenase 260

Hemoproteins: Classification and Behavior of Heme in Absence of Globins 267

Myoglobin and Hemoglobin 275

Myoglobin 275

Hemoglobin 280

Cytochrome C 298

Electron Transfer in Porphyrins and Metalloporphyrins 301

Catalases 311

Peroxidases 315

Cytochrome P-450 322

Electronic Spectra of Hemoproteins 327

ESR Spectra of Hemoproteins 362

References 378

6 Vitamin B_12 393

References 405

7 Chlorophyll 407

References 421

Index 423

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