Forest plays an important role in maintenance of ecosystem equilibrium and provides material energy. It has a set of mechanism of its own growth, development, and metabolism. Proteomics is a crucial means to reveal the material life phenomenon in biology nowadays. In this chapter, the trend of technical system of proteomics, such as extraction, separation, identification method of protein, was introduced and existing problem was indicated. In addition, different research direction of forest proteomics (i.e. tress response, wood formation, developmental regulation, pest control) were discussed respectively combined with overseas research. Finally, the prospect to its future development was given.

PREFACE

Chapter 1: Advances in Proteomics Research on Forest Tree 1

1.1 Introduction 1

1.2 Research progress on proteomic technology system 1

1.3 The question in current proteomics research 3

1.4 Proteomics applications on forestry 4

1.5 Conclusion and prospect 8

References 10

Chapter 2: Comparison of Methods for Protein Extraction from Pine Needles 14

2.1 Introduction 14

2.2 Materials and methods 15

2.3 Results 17

2.4 Discussion 19

References 20

Chapter 3: Temporal and Spatial Profiling of lnternod Elongation-Associated Protein Expression in Rapidly Growing Culms of Bamboo 21

3.1 Introduction 21

3.2 Materials and methods 22

3.3 Results 26

3.4 Discussion 41

3.5 Conclusion 49

References 51

Chapter 4: Physiological and Protein Responses to Drought in Four Pine Seedlings 54

4.1 Introduction 54

4.2 Material and methods 55

4.3 Results and Discussion 59

References 68

Chapter 5: Proteins Respondin g to Drough t and High-Temperatu re Stress in Pinus armandii Franch 72

5.1 Introduction 72

5.2 Mater ial and methods 73

5.3 Results and Discussion 76

5.4 Concluding remarks 83

References 84

Chapter 6: Proteins Responding to Drought and High-Temperature Stress in Populus x euramericana CV. '74/76' 87

6.1 Introduction 87

6.2 Materials and methods 88

6.3 Results 91

6.4 Discussion 96

6.5 Conclusions remarks 98

References 100

Chapter 7: Physiological and Protein Responses to Drought Stress in Hippophae rhamnoides 104

7.1 Introduction 104

7.2 Material and method 105

7.3 Results 108

7.4 Discussion 117

7.5 Conclusion 119

References 120

Chapter 8: Clonal Reproduction and Natural Variation of Populus canescens Patches 122

8.1 Introduction 122

8.2 Materials and methods 123

8.3 Results 127

8.4 Discussion 137

References 140

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