"Approaches to research on the systematics of fish-borne trematodes"is aimed to serve as a guide book for the student in Parasitology,Biology, or Molecular Biology, or for any young researcher who is interested in studying fish-borne trematodes, in particular on their morphological identification and genetic makeup.
In-depth knowledge of medically important fish-borne trematodes is

Preface vii

Chapter 1 Medically Important Fish-Borne Zoonotic Trematodes 1

1.1 Major Fish-Borne Trematodes 2

1.2 Other Fish-Borne Trematodes 10

References 12

Chapter 2 Collection of Fish-Borne Trematodes from the Final Host 17

2.1 Diagnostic Methods 17

2.2 Treatment and Purgation 20

2.3 Collection of Adult FBT from Animals 21

2.4 Worm Preparation for Morphological Study 22

2.5 Diagnostic Characteristics of FBT 22

References 35

Chapter 3 Collection of Fish-Borne Trematode Cercaria 39

3.1 Snail Host of Fish-Borne Trematodes 39

3.2 Cercaria Morphological Study 40

3.3 Morphology of Fresh Cercaria 42

3.4 Vital Stain 43

3.5 Chaetotaxy 43

3.6 Polymerase Chain Reaction 44

References 47

Chapter 4 Collection of Fish-Borne Trematodes in Infective Stage from the Fish: The Second Intermediate Host 49

4.1 Development of Metacercariae 49

4.2 Methods of Fish Examination for Metacercariae 50

4.3 Mobile Laboratory for Fish Examination 50

4.4 Method of Excysting Metacercariae 51

4.5 Morphology of Metacercariae 54

References 60

Chapter 5 Molecular Systematics of Fish-Borne Trematodes 61

5.1 What is Molecular Systematics? 61

5.2 Species Identification: Morphological and Molecular Approaches 62

5.3 Advantages and Disadvantages of using DNA Sequences for Systematics 62

5.4 Molecular Markers 63

5.5 The Regions of DNA used for Molecular Systematics 63

5.6 Molecular Systematics of Fish-Borne Trematodes 67

5.7 Summary and Future Direction 71

References 73

Chapter 6 Methods of Molecular Study: DNA Sequence and Phylogenetic Analyses 77

6.1 Criterion for Selecting Appropriate Genetic Markers 77

6.2 Phylogenetic Analysis 80

6.3 How to Construct Phylogenetic Trees 83

6.4 Further Perspectives 86

References 88

Appendix A 91

Appendix B 105

Glossary 109

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